Affilliation: Shenzhen Institutes of Advanced Technology;
Chinese Academy of Sciences
Yi Lu, PhD, Associate Professor, Director of Shenzhen Engineering Lab for Brain Activity Mapping Technologies. Dr. Lu received his PhD degree from Wuhan University in 2010 on the study of the neural electrodes. He then joined Shenzhen Institutes of Advanced Technologies, Chinese Academy of Sciences (SIAT, CAS). His current research interests focus on developing novel research tools for brain activity mapping and dissecting the function of specific neural circuits in neuropsychological diseases. Dr. Lu has published 12 research papers, he also holds 19 Chinese patents and 25 patent applications.
Talk Title: Novel optrode arrays and their applications in neural circuit dissection
The field of optogenetics has been successfully used to understand the mechanisms of neuropsychiatric diseases through the precise spatial and temporal control of specific groups of neurons in a neural circuitry. However, it remains a great challenge to integrate optogenetic modulation with electrophysiological, and behavioral read out methods as a means to explore the causal, temporally precise, and behaviorally relevant interactions of neurons in the specific circuits of freely behaving animals. Therefore, we developed multi-functional optrodes for drug delivery, optical stimulation, and electrical recordings in multiple brain regions in vivo. The optrodes were integrated with other modification methods to build a comparatively more advanced electrode/neural-tissue interface. With the aid of these optrodes, we then preformed precisely timed, cell type-specific optogenetic methodologies to dissect the direction of ictal propagation in vivo. We proved that the Dentate Gyrus/Hilus (DGH) dominates the propagation of ictal discharges. We also demonstrated that cyclically activate the DGH interneurons shows a long-lasting inhibitory effect on ictal seizures, significantly reducing the frequency of behavioral seizures in freely moving animals, which may be a potential therapeutic target for Temporal Lobe Epilepsy.